Dynamics of distribution and structural-functional features of mast cells in the thyroid gland of rat's offspring in the norm and after penatal exposue of staphylococcal toxoid
Vol 90 No 1 (2021), Theoretical and Experimental Medicine
Vol 90 No 1 (2021)

Dynamics of distribution and structural-functional features of mast cells in the thyroid gland of rat's offspring in the norm and after penatal exposue of staphylococcal toxoid

Theoretical and Experimental Medicine
https://doi.org/10.35339/ekm.2021.90.1.fov
Published March 31, 2021
O.V. Fedosieieva
Zaporizhia State Medical University, Zaporizhia, Ukraine
https://orcid.org/0000-0003-2247-1965
PDF (Українська)

Keywords

thyroid gland
antigen
staphylococcal toxoid
mast cells
experiment
rats

How to Cite

Fedosieieva, O. (2021). Dynamics of distribution and structural-functional features of mast cells in the thyroid gland of rat’s offspring in the norm and after penatal exposue of staphylococcal toxoid. Experimental and Clinical Medicine, 90(1), 5-12. https://doi.org/10.35339/ekm.2021.90.1.fov
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Abstract

The quantitative and functional state of mast cells, features of their localization in the age aspect of the early postnatal period of ontogenesis in normal and after prenatal exposure of staphylococcal toxoid to the fetus were studied in the thyroid gland of rats. As a material of the study were thyroid glands of Wistar rats aged 3 to 30 days of postnatal development (108 animals). Three groups of animals on the 3rd, 7th, 11th, 14th, 21st, 30th days of the postnatal life period were studied: I group – intact animals (norm); ІІ group – control, animals which were injected with 0.9% NaCl solution on the 18th day of the dated pregnancy; III group – experimental animals injected with staphylococcal toxoid liquid purified adsorbed (10-14 binding units in 1 ml, diluted 10 times) at a dose of 0.05 ml. Staphylococcal toxoid and 0.9% NaCl solution were administered to the fetus surgically during laparotomy, by intrauterine, transdermal subcutaneous injection into the interscapular area at a dose of 0.05 ml to each fetus. It is established that as a result of prenatal action of antigen-staphylococcal toxoid on the body postnatally in the early stages of development in the thyroid gland there is a balance between the processes of migration, distribution, degranulation of mast cells and proliferative processes of parenchyma, gland stroma and lymph node formation. In the thyroid gland during morphogenetic processes with the appearance of new follicles and intracellular regeneration, the population of mast cells responds with active degranulation, with the release into the intercellular space of biologically active substances, which apparently have a modulating effect on thyroid components and supporting the thyroid gland. recovery processes.

Keywords: thyroid gland, antigen, staphylococcal toxoid, mast cells, experiment, rats.

https://doi.org/10.35339/ekm.2021.90.1.fov
PDF (Українська)

References

Jonsdottir B, Lundgren M, Wallengren S, Lernmark Å , Jönsson I, et al. Are Perinatal Events Risk Factors for Childhood Thyroid Autoimmunity? European thyroid journal. 2017;6(6):298-306. DOI: 10.1159/000479964. PMID: 29234623. PMCID: PMC5704722.

Liu P, Zheng J, Yang P, Wang X, Wei Co, Zhang S, et al. The immunologic status of newborns born to SARS-CoV-2-infected mothers in Wuhan, China. J. Allergy Clin. Immunol. 2020;146(1):101-9.e1. DOI: 10.1016/j.jaci.2020.04.038. PMID: 32437740. PMCID: PMC7211641.

Ajjan RA, Weetman AP. The pathogenesis of Hashimotos thyroiditis: further developments in our understanding. Horm. Metab. Res. 2015;47(10):702-10. DOI: 10.1055/s-0035-1548832. PMID: 26361257.

Da Silva E, Jamur M, Oliver C. Mast cell function: a new vision of an old cell. J. Histochem. Cytochem. 2014;62(10):698-738. DOI: 10.1369/0022155414545334. PMID: 25062998. PMCID: PMC4230976.

Gilfillan AM, Beaven MA. Regulation of mast cell responses in health and disease. Crit. Rev. Immunol. 2011;31(6):475-529. DOI: 10.1615/critrevimmunol.v31.i6.30. PMID: 22321108 PMCID: PMC3395887.

Brown MA, Hatfield JK. Mast Cells are Important Modifiers of Autoimmune Disease: With so Much Evidence, Why is There Still Controversy? Front. Immunol. 2012;3:147. DOI: 10.3389/fimmu.2012.00147. PMID: 22701454. PMCID: PMC3369183.

Xu Y, Chen G. Mast cell and autoimmune diseases. Mediators Inflamm. 2015;2015:246126. DOI: 10.1155/2015/246126. PMID: 25944979. PMCID: PMC4402170.

Lu LF, Lind EF, Gondek DC, Bennett KA, Gleeson MW, Pino-Lagos K, Scott ZA, et al. Mast cells are essential intermediaries in regulatory T-cell tolerance. Nature. 2006;442(7106):997-1002. DOI: 10.1038/nature05010. PMID: 16921386.

Kambayashi T, Kambayashi T, Allenspach EJ, Chang JT, Zou T, Shoag JE, Reiner SL, et al. Inducible MHC class II expression by mast cells supports effector and regulatory T cell activation. J. Immunol. 2009;182(8):4686-95. DOI: 10.4049/jimmunol.0803180. PMID: 19342644. PMCID: PMC2922395.

Mahinko VI, Nikitin VN. Konstanty rosta i funkcionalnye periody razvitiia v postnatalnoi zhizni belyh krys [Growth constants and functional periods of development in the postnatal life of white rats]. Kiev: Naukova dumka; 1975. Molekuliarnye i fiziologicheskie mehanizmy vozrastnogo razvitiia [Molecular and physiological mechanisms of age development]; p. 308-25 [in Russian].

Medic VA, Tokmachev MS, Fishman BB. Statistika v medicine i biologii [Statistics in medicine and biology]. In 2 volumes. Komarov YM, editor. Moscow: Medicine; 2000. 412 p. Volume 1, Teoreticheskaja statistika [Theoretical statistics]. [In Russian].

Fedosieieva ОV. Morphogenesis of rat’s thyroid gland in preweaning period after prenatal influence of staphylococcal toxoid. Svit medytsyny ta biolohiyi – World of Medicine and Biology. 2020;3(73):230-4.