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Abstract
The aim of this study was to assess the viability of human sperm after cryopreservation with non-penetrating cryoprotectants that do not require removal from the suspension cells before using spermatozoa in ART. As a result of the studies, it was found that using of sucrose and PVP in various combinations as cryoprotectant agents for sperm freezing allows there to achieved 60–80 % of their survival rate. Thus, cryopreservation of human sperm using sucrose and PVP is promising for ART, since sperm can be used immediately after warming to fertilize oocytes in vitroReferences
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