Keywords
nanoparticles
betamethasone
superoxide dismutase
highly selective inhibitor of iNOS 1400 W
How to Cite
Abstract
It was demonstrated in experiment that profound structural changes of nuclei and cytoplasm are observed in contact nickel dermatitis. Pyknosis of nuclei, and its shrinking was seen, karyoplasm’s osmiophilia and injury of nucleoli membranes were observed also. Organelles were damaged, lysis of tonofilaments was fixed. In necrotic areas of skin derma components of microcirculation were destroyed. Application of nano-encapsulated medications of pegylated superoxide dismutase, highly selective inhibitor of iNOS 1400 W and betamethasone propionate causes skin healing and restoring of layers structure of epidermis, its differentiation, keratinization and the formation of horny scales. Epidermocytes of basal and spinous layers include rounded nuclei with clearly contoured karyolemma. Cytoplasm contains a lot of microfilaments, organelles are unchanged. Perivascular edema is absent. Skin trophic is normalized. Polymeric nanoparticles with encapsulated anti-inflammatory and anti-oxidizing medications and NO synthase inhibitors for transdermal delivery are the prospective direction for further investigation and clinical implementation for treatment of contact dermatitis.References
Ramirez F., Chren M.-M., Botto N. (2017). A review of the impact of patch testing on quality of life in allergic contact dermatitis. Journal of the American Academy of Dermatology. 76(5): 1000–1004.
Lim H.W., Collins S.A.B., Resneck J.S. et al. (2017). The burden of skin disease in the United States. Journal of the American Academy of Dermatology. 76(5): 958–972.e2.
Zadymova N. (2013). Kolloydno-khymycheskye aspekty transdermalnoy dostavky lekarstv (obzor). Kolloydnyy Zhurnal. 75(5): 543–556 [in Russian]
Simpson E.L., Chalmers J.R., Hanifin J.M. et al. (2014). Emollient enhancement of the skin barrier from birth offers effective atopic dermatitis prevention. Journal of Allergy and Clinical Immunology. 134(4): 818–823.
Misery L. (2011). Atopic dermatitis and the nervous system. Clinical Reviews in Allergy & Immunology. 41(3): 259–266.
Shandra O., Shukhtin V. (2015). Atopycheskyy dermatyt y vzaymodeystvye nervnoy, éndokrynnoy y ymmunnoy system. Dermatolohiya ta Venerolohiya. (2): 30–41.
Chekman I.S. (2009). Nanochastynky: vlastyvosti ta perspektyvy zastosuvannya. Ukrayinskyy Biokhimichnyy Zhurnal. 81(1): 122–128 [in Ukrainian].
Hussain Z., Katas H., Mohd Amin M.C.I. et al. (2013). Self-assembled polymeric nanoparticles for percutaneous co-delivery of hydrocortisone/hydroxytyrosol: An ex vivo and in vivo study using an NC/Nga mouse model. International Journal of Pharmaceutics. 444(1–2): 109–119.
Schieber M., Chandel N. (2014). ROS function in redox signaling and oxidative stress. Current Biology. 24(10): 453–462.
Ross R., Reske-Kunz A.B. (2001). The role of NO in contact hypersensitivity. International Immunopharmacology. 1(8): 1469–1478.
Coondoo A. (2014). Topical corticosteroid misuse: The Indian Scenario. Indian Journal of Dermatology. 59(5): 451.
Khudan-Tsilo I.I., Korda M.M. (2017). Nano-forma preparatu superoksyddysmutazy – perspektyvnyy metod likuvannya kontaktnoho dermatytu. Visnyk Naukovykh Doslidzhen. (3): 144–148 [in Ukrainian].
Khudan-Tsilo I.I., Korda M.M. (2017). Strukturni zminy shkiry pry kontaktnomu nikelevomu dermatytovi za umov pryhnichennya iNOS. Zdobutky Klinichnoyi I Eksperymental’noyi Medytsyny. 1(3): 165–169 [in Ukrainian].
Horal’s’kyy L., Khomych V.T. (2015). Osnovy histolohichnoyi tekhniky i morfofunktsionalni metody doslidzhen u normi ta pry patolohiyi. (L.P. Horal’s’kyy, Ed.) (3rd ed.). Zhytomyr: Polissya [in Ukrainian].