Keywords
placental blood serum
cryopreservation
mild traumatic brain injury
anxiety
elevated plus maze test
anxiolytic activity
How to Cite
Abstract
In press
Background. The significant prevalence of traumatic brain injury during armed conflicts and the insufficient effectiveness of existing treatment programs for victims necessitates the development of new therapeutic approaches.
Aim. To investigate the effect of cryopreserved human placental blood serum on the level of anxiety in mice with a model of mild blast-induced traumatic brain injury.
Materials and Methods. Human placental blood was obtained from the umbilical vein of postpartum placenta weeks of gestation with a physiological course of pregnancy. Experiments were conducted on 6-month-old mice. Human placental blood was obtained from the umbilical vein of postpartum placenta 38–40 weeks of gestation with a physiological course of pregnancy. Experiments were conducted on 6-month-old mice. Statistical analysis of the obtained experimental data was performed by analysis of variance using the software package "StatGraphics Plus 2.1" (Statgraphics Technologies, Inc., USA). The study was carried out within the framework of the scientific project "Cryotechnologies in overcoming the consequences of combat blast-induced brain injury", state registration number 0125U000872.
Research Ethics. Human placental blood was obtained with informed consent of parturient women in compliance with the principles of the World Medical Association Declaration of Helsinki (1964–2024). All manipulations with animals were carried out in accordance with the "General Principles of Animal Experiments" adopted by the First National Congress on Bioethics (Kyiv, 2001) and consistent with the provisions of the "Convention of the Council of Europe for the Protection of Laboratory Animals" (ETS 123, 1986).
Results. Analysis of the obtained data proves that mild blast-induced craniocerebral trauma leads to increased anxiety in mice. Conducting a 5-day course of injections of cryopreserved or cryosublimated cord blood serum in the first days after the injury reduces pathological anxiety of experimental animals, which demonstrated a lower percentage of the total time spent in the closed arms of the maze, lower coefficients of preference for visiting and staying in the closed arms of the maze, a longer average duration of one episode of staying on the platform between the arms of the maze and higher exploratory activity. Cryosublimated cord blood serum was not inferior in activity to cryopreserved
Conclusions. The revealed anxiolytic effect of the use of cryopreserved, cryosublimated placental blood serum allows us to consider it as a promising means of overcoming increased anxiety – a pathological consequence of mild blast-induced craniocerebral trauma.
Keywords: neurology, placental blood serum, cryopreservation, mild traumatic brain injury, anxiety, elevated plus maze test, anxiolytic activity.
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