Keywords
8-isoprostanes
catalase
antioxidants
How to Cite
Abstract
In press
The aim of study was to evaluate the effect of water saturated with molecular hydrogen on the content of 8-isoprostanes and catalase activity in blood serum and colon wall tissue of rats with CRC treated with 5-fluorouracil. Experiments were conducted on 90 male Wistar white rats. CRC was simulated in animals by subcutaneous injection of 1,2-dimethylhydrazine at a dose of 7.2 mg/kg once a week for 30 weeks. Rats consumed water saturated with molecular hydrogen at a concentration of 0.6 ppm ad libitum. In biological material the content of 8-isoprostanes was measured by immunoassay method and the level activity of catalase was measured by the spectrophotometric method. The content of 8-isoprostanes in blood serum and colon tissue of rats with CRC was 94,5 and 474.4% higher than in control animals, while catalase activity in serum increased by 86.6% and in intestinal wall decreased by 50.7%. In rats that consumed water enriched with molecular hydrogen for 30 weeks, the content of 8-isoprostanes in the blood serum decreased by 36,2% and in the intestinal wall by 51.2%, compared to animals with CRC that were not corrected. Catalase activity under the influence of water enriched with molecular hydrogen decreased in the intestines by 41.6% compared to the corresponding indicators in rats not corrected with molecular hydrogen. The use of chemotherapy (5-fluorouracil) in animals with CRC leads to an even greater increase in the intensity of oxidative stress in blood serum and the intestinal wall. Ad libitum access of CRC rats receiving chemotherapy to molecular hydrogen-enriched water resulted in a significant decrease in 8-isoprostane levels in both biological materials and an increase in catalase activity in the intestine wall. The study shows that consumption of water saturated with molecular hydrogen by animals with CRC leads to a significant improvement in the indicators of the intensity of antioxidant system.
Keywords: hydrogen rich water, 8-isoprostanes, catalase, antioxidants.
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